5D0I

Structure of RING finger protein 165

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-28
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	76.220, 76.220, 103.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.369 - 1.900
R-factor	0.1747
Rwork	0.173
R-free	0.19980
RMSD bond length	0.008
RMSD bond angle	1.013
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.370
High resolution limit [Å]	1.900
Number of reflections	23021
<I/σ(I)>	14.8
Completeness [%]	94.3
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	HEPES, sodium chloride, ammonium sulfate, ethylene glycol