5CZP

70S termination complex containing E. coli RF2

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-01
Detector	PSI PILATUS 6M
Wavelength(s)	0.97890
Spacegroup name	P 21 21 21
Unit cell lengths	210.890, 450.120, 620.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.921 - 3.300
R-factor	0.267434023318
Rwork	0.267
R-free	0.28645
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.008
RMSD bond angle	0.846
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.930	49.930	3.390
High resolution limit [Å]	3.300	14.760	3.300
Rmerge	0.272	0.053	2.079
Rmeas	0.301	0.058	2.319
Total number of observations	4668537
Number of reflections	866742	9599	64001
<I/σ(I)>	5.26	19.39	0.84
Completeness [%]	98.9	91.5	99.2
CC(1/2)	0.994	0.998	0.213

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	4.9% PEG 20k, 9.8% PEG 200, 0.1M TRIS-ACETATE, 0.2M KSCN, 10mM MAGNESIUM ACETATE