5CZO
Structure of S. cerevisiae Hrr25:Mam1 complex, form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2829 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.701, 83.841, 132.508 |
| Unit cell angles | 90.00, 92.57, 90.00 |
Refinement procedure
| Resolution | 49.692 - 2.894 |
| R-factor | 0.2197 |
| Rwork | 0.218 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xhl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.505 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.400 | 2.950 |
| High resolution limit [Å] | 2.890 | 2.900 |
| <I/σ(I)> | 15.4 | 1.36 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.1M Imidazole pH 8.0, 14% PEG3350, 6% Glycerol |
