5CZJ

Crystal structure of HypD, a 1-pyrroline-4-hydroxy-2-carboxylate deaminase from Sinorhizobium meliloti

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-10-16
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 3
Unit cell lengths	124.865, 124.865, 124.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.431 - 1.920
R-factor	0.2855
Rwork	0.283
R-free	0.33820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hmc
RMSD bond length	0.004
RMSD bond angle	0.737
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.431	1.950
High resolution limit [Å]	1.920	1.920
Rmerge	0.071	0.583
Number of reflections	49734
<I/σ(I)>	36.88
Completeness [%]	100.0	100
Redundancy	21.3	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.5 microL of 19.5 mg/mL protein solution (including 20% (w/v) glycerol) mixed with 0.5 microL of reservoir solution (0.2 M potassium/sodium tartrate, 20% (w/v) PEG3350).