5CZE
Crystal structure of the PaaA2-ParE2 antitoxin-toxin complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 143.226, 143.226, 86.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.880 - 3.820 |
| R-factor | 0.256 |
| Rwork | 0.253 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cw7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.880 | 3.960 |
| High resolution limit [Å] | 3.822 | 3.820 |
| Rmerge | 0.102 | 0.494 |
| Number of reflections | 9903 | |
| <I/σ(I)> | 12.91 | 3.1 |
| Completeness [%] | 97.1 | 83.7 |
| Redundancy | 7.8 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 20 mM calcium chloride, 100 mM sodium acetate pH 4.6, 30% MPD |
