5CZD
The complex structure of VinK with VinL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.140, 68.230, 148.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.070 - 2.340 |
| R-factor | 0.20528 |
| Rwork | 0.202 |
| R-free | 0.27287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5czc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.627 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.230 | 2.460 |
| High resolution limit [Å] | 2.337 | 2.337 |
| Rmerge | 0.140 | 0.758 |
| Number of reflections | 19598 | |
| <I/σ(I)> | 9.1 | 2.1 |
| Completeness [%] | 99.3 | 97.4 |
| Redundancy | 6.3 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 278 | 0.05 M magnesium chloride, 20% polyethylene glycol 8000, 0.1 M Tris-HCl |
