5CYZ
Structure of S. cerevisiae Hrr25:Mam1 complex, form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.190, 83.986, 67.424 |
| Unit cell angles | 90.00, 108.53, 90.00 |
Refinement procedure
| Resolution | 48.200 - 1.841 |
| R-factor | 0.1647 |
| Rwork | 0.163 |
| R-free | 0.19210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xhl |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.016 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 1.880 |
| High resolution limit [Å] | 1.840 | 1.850 |
| Number of reflections | 48597 | |
| <I/σ(I)> | 17 | 1.4 |
| Completeness [%] | 99.0 | 98 |
| Redundancy | 6.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 293 | 0.1M CHES pH8.9, 5% Tacsimate, 25 mM YCl3, 19% PEG3350 |
