5CYY

Structure of the C-terminal domains of DipZ from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-01-01
Detector	ADSC QUANTUM 4r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	109.972, 117.525, 122.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.393 - 2.200
R-factor	0.1872
Rwork	0.182
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.088
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	99.000	99.000	2.280
High resolution limit [Å]	2.200	4.740	2.200
Rmerge	0.067	0.043	0.628
Rmeas	0.135	0.049
Rpim	0.067	0.023	0.628
Total number of observations	302594
Number of reflections	73550
<I/σ(I)>	7.8		1.57
Completeness [%]	90.1	85.2	89.7
Redundancy	4.1	4.4	2.9
CC(1/2)		0.997	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG4000, 0.1M Na-citrate pH 5.6