5CYY
Structure of the C-terminal domains of DipZ from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-01-01 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 109.972, 117.525, 122.438 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.393 - 2.200 |
R-factor | 0.1872 |
Rwork | 0.182 |
R-free | 0.23710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.088 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 99.000 | 99.000 | 2.280 |
High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
Rmerge | 0.067 | 0.043 | 0.628 |
Rmeas | 0.135 | 0.049 | |
Rpim | 0.067 | 0.023 | 0.628 |
Total number of observations | 302594 | ||
Number of reflections | 73550 | ||
<I/σ(I)> | 7.8 | 1.57 | |
Completeness [%] | 90.1 | 85.2 | 89.7 |
Redundancy | 4.1 | 4.4 | 2.9 |
CC(1/2) | 0.997 | 0.611 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20% PEG4000, 0.1M Na-citrate pH 5.6 |