5CYW
Crystal Structure of Vaccinia Virus C7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97948 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 100.653, 100.653, 100.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.013 - 2.000 |
| R-factor | 0.1546 |
| Rwork | 0.153 |
| R-free | 0.18220 |
| Structure solution method | SAD |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.656 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.545 | |
| Number of reflections | 22967 | |
| <I/σ(I)> | 50.8 | 1.8 |
| Completeness [%] | 98.7 | 86.6 |
| Redundancy | 19.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris, pH 8.5, 3.5 M sodium chloride |
