5CYP

GTPase domain of Septin 9 in complex with GTP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	74.587, 79.156, 108.217
Unit cell angles	90.00, 100.38, 90.00

Refinement procedure

Resolution	45.654 - 2.893
R-factor	0.2544
Rwork	0.252
R-free	0.29740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.594
Data reduction software	MOSFLM
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		3.070
High resolution limit [Å]	2.890	8.550	2.890
Rmerge	0.121	0.026	0.652
Rmeas	0.145	0.032	0.789
Total number of observations	85852
Number of reflections	27269	1083	4266
<I/σ(I)>	8.57	28.22	1.9
Completeness [%]	97.6	95.1	95.2
Redundancy	3.25

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	291	20% PEG1500, 20% Glycerol