5CYJ
X-ray structure of human RBPMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 83.482, 90.944, 47.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.500 - 1.790 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.23900 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.292 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.500 | 1.890 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.098 | 0.900 |
| Number of reflections | 17458 | |
| <I/σ(I)> | 17 | 2.2 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 8.5 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 0.2 M lithium sulfate, 0.1M Tris-HCl pH 8.5, 40% (v/v) PEG 400 |
