5CYA

Crystal structure of Arl2 GTPase-activating protein tubulin cofactor C (TBCC)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.9795
Spacegroup name	P 43
Unit cell lengths	69.790, 69.790, 78.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.105 - 2.000
R-factor	0.203
Rwork	0.201
R-free	0.23830
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.001
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	SOLVE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.900	34.105	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge	0.082	0.037	0.716
Rmeas	0.088
Rpim	0.032	0.015	0.279
Total number of observations	193620	6190	27935
Number of reflections	25341
<I/σ(I)>	13.4	34.2	2.7
Completeness [%]	100.0	99.5	100
Redundancy	7.6	7.4	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	0.1 M Sodium Citrate, 0.4 M Ammonium Sulfate, 0.7 M Lithium Sulfate pH 5.2