5CY7
Structure of Xoo1075, a peptide deformylase from Xanthomonas oryze pv oryze, in complex with fragment 275
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 58.823, 58.823, 264.034 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.400 - 2.400 |
| R-factor | 0.18216 |
| Rwork | 0.181 |
| R-free | 0.21243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e5d |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.290 | |
| Number of reflections | 11015 | |
| <I/σ(I)> | 35.3 | 7.4 |
| Completeness [%] | 95.2 | 88 |
| Redundancy | 9.2 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.05M Cadmium sulfate, 0.1M HEPES, 2.0M Sodium acetate trihydrate, pH 7.5 |
