5CXM
Crystal structure of the cyanobacterial plasma membrane Rieske protein PetC3 from Synechocystis PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-11 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 1 |
| Unit cell lengths | 21.747, 55.011, 80.709 |
| Unit cell angles | 80.44, 89.87, 84.11 |
Refinement procedure
| Resolution | 26.530 - 1.700 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e74 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 38205 | |
| <I/σ(I)> | 15.1 | 2.5 |
| Completeness [%] | 94.8 | 61.1 |
| Redundancy | 2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.15 | 9mM NiSOx6H2O, 90mM Tris 22.5% (w/v) PEG-MME 2K, 10mM spermidine |
