5CX0
Structure of Xoo1075, a peptide deformylase from Xanthomonas oryzae pv. oryzae, in complex with fragment 322
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6C1 |
Synchrotron site | PAL/PLS |
Beamline | 6C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.99 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 58.751, 58.751, 266.092 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.400 - 2.500 |
R-factor | 0.19431 |
Rwork | 0.192 |
R-free | 0.23816 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.991 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 11485 |
<I/σ(I)> | 12.3 |
Completeness [%] | 98.8 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.05M Cadmium sulfate, 0.1M HEPES, 2.0M Sodium acetate trihydrate, pH 7.5 |