5CWW

Crystal structure of the Chaetomium thermophilum heterotrimeric Nup82 NTD-Nup159 TAIL-Nup145N APD complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	122.300, 107.960, 69.590
Unit cell angles	90.00, 108.65, 90.00

Refinement procedure

Resolution	29.187 - 2.200
R-factor	0.1865
Rwork	0.185
R-free	0.21310
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.756
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Number of reflections	43372
<I/σ(I)>	13.6
Completeness [%]	99.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	100 mM lithium citrate 20 % (w/v) PEG 3,350 3 % (v/v) MPD