5CWV

Crystal structure of Chaetomium thermophilum Nup192 TAIL domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 21 21 21
Unit cell lengths	64.745, 114.474, 114.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.891 - 3.155
R-factor	0.1987
Rwork	0.194
R-free	0.23660
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.650
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.150
Number of reflections	13043
<I/σ(I)>	21.8
Completeness [%]	99.5
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	294	50 mM HEPES, pH 7.1 15 % (w/v) PEG 200 340 mM sodium chloride