5CW7

Crystal structure of the PaaA2-ParE2 antitoxin-toxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97911
Spacegroup name	P 41 21 2
Unit cell lengths	90.540, 90.540, 412.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.369 - 2.827
R-factor	0.1877
Rwork	0.185
R-free	0.23990
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.252
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHELX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.370	2.928
High resolution limit [Å]	2.827	2.827
Number of reflections	42331
<I/σ(I)>	23.91
Completeness [%]	99.8
Redundancy	25.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	100 mM magnesium chloride, 100 mM MES pH 6.1, 8% PEG 6000