5CW4

Structure of CfBRCC36-CfKIAA0157 complex (Selenium Edge)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	50.106, 116.287, 231.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.440 - 2.543
R-factor	0.1901
Rwork	0.189
R-free	0.24010
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.554
Data scaling software	XDS
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.440	11.370	2.610
High resolution limit [Å]	2.540	8.040	2.540
Rmerge	0.048	0.019	1.169
Rmeas	0.059	0.023	1.425
Total number of observations	243850
Number of reflections	84216	1736	6020
<I/σ(I)>	13.74	41.13	1.02
Completeness [%]	98.1	98.8	95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Na HEPES (pH 7.0), 10% w/v PEG4000 and 10% (v/v) 2-propanol