5CVV
coniferyl alcohol bound monolignol 4-O-methyltransferase 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.290, 67.480, 74.070 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 73.960 - 1.730 |
| R-factor | 0.18284 |
| Rwork | 0.181 |
| R-free | 0.21249 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.250 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 73.960 |
| High resolution limit [Å] | 1.730 |
| Number of reflections | 70249 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 100.0 |
| Redundancy | 5.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | MOMT9 was grown via a hanging-drop vapor diffusion method in 22% (w/v) PEG 4000, 0.3 M Mg(NO3)2 (pH 7.2), and 1 mM DTT with 1 mM SAH and 1 mM coniferyl alcohol |
