5CVL
WDR48 (UAF-1), residues 2-580
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.026382 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 139.520, 139.520, 235.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.330 - 3.000 |
| R-factor | 0.1901 |
| Rwork | 0.186 |
| R-free | 0.23290 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Refinement software | BUSTER-TNT (2.11.5) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.123 | 0.041 | 0.827 |
| Total number of observations | 381541 | ||
| Number of reflections | 44419 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 8.6 | 8.5 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 0.72 M sodium phosphate, 0.72 M potassium phosphate, 90 mM HEPES pH 7.5 |
