5CV9
Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/V66H at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-09-25 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.116, 60.283, 38.342 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 38.260 - 1.620 |
| R-factor | 0.1846 |
| Rwork | 0.183 |
| R-free | 0.22030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.834 |
| Data reduction software | APEX |
| Data scaling software | APEX (2013/1 for Windows) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.017 | 0.121 |
| Total number of observations | 320076 | |
| Number of reflections | 18026 | |
| <I/σ(I)> | 39.7 | 6.67 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17.62 | 5.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
