5CV8
Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/T62H at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-04-27 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.194, 60.236, 38.490 |
| Unit cell angles | 90.00, 94.19, 90.00 |
Refinement procedure
| Resolution | 31.110 - 1.580 |
| R-factor | 0.1596 |
| Rwork | 0.157 |
| R-free | 0.19790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.784 |
| Data reduction software | APEX |
| Data scaling software | APEX |
| Phasing software | PHASER (2.5.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.020 | 0.168 |
| Number of reflections | 19546 | |
| <I/σ(I)> | 29.76 | 5.57 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.83 | 7.99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 21% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
