5CV5
Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-07-18 |
| Detector | APEX II CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.831, 60.549, 77.901 |
| Unit cell angles | 90.00, 93.15, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| R-factor | 0.1869 |
| Rwork | 0.185 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.792 |
| Data reduction software | APEX |
| Data scaling software | APEX (2008/2 for Windows) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 60.550 | 1.840 |
| High resolution limit [Å] | 1.800 | 4.960 | 1.800 |
| Rmerge | 0.026 | 0.037 | 0.234 |
| Total number of observations | 510698 | ||
| Number of reflections | 26668 | 1338 | 1291 |
| <I/σ(I)> | 29.29 | 85.12 | 4.24 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 18.84 | 27.3 | 7.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 25% MPD, 25 mM potassium phosphate, calcium chloride, pdTp |
