5CV0
Crystal structure of N-terminal truncated human B12-chaperone CblD (108-296)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03299 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.638, 67.111, 66.219 |
| Unit cell angles | 90.00, 110.54, 90.00 |
Refinement procedure
| Resolution | 31.390 - 1.900 |
| R-factor | 0.18811 |
| Rwork | 0.186 |
| R-free | 0.22722 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cuz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.392 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.417 |
| Number of reflections | 30281 | |
| <I/σ(I)> | 13.1 | 2.5 |
| Completeness [%] | 98.1 | 97 |
| Redundancy | 3.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20 % PEG3350, 0.1M Tris-HCl, pH 7.5, 0.2 M MgCl2, 0.1 M NaF |
