5CUO

Structure of Rhodopseudomonas palustris PduL - CoA bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	57.740, 56.443, 150.438
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.442 - 1.544
R-factor	0.1896
Rwork	0.189
R-free	0.22050
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.442	1.600
High resolution limit [Å]	1.540	1.540
Rmerge	0.169	1.223
Number of reflections	72720
<I/σ(I)>	12.9	1.7
Completeness [%]	99.4	94.4
Redundancy	13.9	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	35 % MPD