5CUF
X-ray crystal structure of SeMet human Sestrin2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97879 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 292.680, 292.680, 292.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.120 - 3.500 |
| R-factor | 0.23715 |
| Rwork | 0.235 |
| R-free | 0.27194 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.824 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.120 | 3.590 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.305 | |
| Number of reflections | 52390 | |
| <I/σ(I)> | 28.5 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 88.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M MES 1.15 M sodium malonate |
