5CTQ

Crystal structure of human SART3/TIP110 half-a TPR (HAT) domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	120.912, 80.529, 146.641
Unit cell angles	90.00, 99.36, 90.00

Refinement procedure

Resolution	38.150 - 2.600
R-factor	0.2364
Rwork	0.235
R-free	0.28140
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.451
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.097	0.395
Number of reflections	87186
<I/σ(I)>	14.5	2.07
Completeness [%]	95.1	82.9
Redundancy	2.5	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200mM sodium formate pH 7.5, 30% PEG4000