5CSR

Crystal structure of triosephosphate isomerase from Thermoplasma acidophilium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-21
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	75.486, 113.963, 114.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.940
R-factor	0.18191
Rwork	0.180
R-free	0.21541
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hg3
RMSD bond length	0.019
RMSD bond angle	1.648
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.940
Number of reflections	73921
<I/σ(I)>	32.4
Completeness [%]	99.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	297	0.5 M sodium chloride, 10% PEG 6000, 0.1 M Tris-HCl