5CSQ
The structure of the NK1 fragment of HGF/SF complexed with MOPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.683, 63.528, 57.528 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 26.290 - 1.950 |
| R-factor | 0.226 |
| Rwork | 0.223 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nk1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.231 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.040 | 0.311 |
| Number of reflections | 27756 | |
| <I/σ(I)> | 20.5 | |
| Completeness [%] | 98.7 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 19% PEG 4000, 200 mM NA Acetate, 150 mM Tris |
