5CSK
Crystal structure of yeast acetyl-CoA carboxylase, unbiotinylated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.100 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 159.879, 159.879, 615.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.470 - 3.100 |
| R-factor | 0.2199 |
| Rwork | 0.217 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AC1-2 AC3-5 BT-BCCP-AC1-5 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.494 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.109 | 0.036 | 0.881 |
| Rmeas | 0.127 | 0.046 | |
| Rpim | 0.085 | 0.029 | 0.680 |
| Total number of observations | 307202 | ||
| Number of reflections | 136014 | ||
| <I/σ(I)> | 6.4 | ||
| Completeness [%] | 93.1 | 92.2 | 90.5 |
| Redundancy | 2.3 | 2.2 | 2.3 |
| CC(1/2) | 0.996 | 0.452 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 293 | 14% (w/v) PEG3350, 4% (v/v) tert-butanol, and 0.2 M sodium citrate |
