5CSA
Crystal structure of domains BT-BCCP-AC1-AC5 of yeast acetyl-CoA carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 93.329, 149.667, 95.437 |
Unit cell angles | 90.00, 118.39, 90.00 |
Refinement procedure
Resolution | 42.630 - 3.000 |
R-factor | 0.23262 |
Rwork | 0.230 |
R-free | 0.28867 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AC1-2 AC3-5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.315 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.088 | 0.039 | 0.449 |
Rmeas | 0.104 | 0.046 | 0.534 |
Rpim | 0.055 | 0.024 | 0.286 |
Total number of observations | 155441 | ||
Number of reflections | 45742 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.2 | 96.3 | 100 |
Redundancy | 3.4 | 3.4 | 3.4 |
CC(1/2) | 0.998 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 80 mM HEPES (pH 7.5), 9.6% (w/v) PEG6000, 1.6% (v/v) MPD, 60 mM sodium citrate, and 80 mM NaI |