5CSA
Crystal structure of domains BT-BCCP-AC1-AC5 of yeast acetyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.329, 149.667, 95.437 |
| Unit cell angles | 90.00, 118.39, 90.00 |
Refinement procedure
| Resolution | 42.630 - 3.000 |
| R-factor | 0.23262 |
| Rwork | 0.230 |
| R-free | 0.28867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AC1-2 AC3-5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.315 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.088 | 0.039 | 0.449 |
| Rmeas | 0.104 | 0.046 | 0.534 |
| Rpim | 0.055 | 0.024 | 0.286 |
| Total number of observations | 155441 | ||
| Number of reflections | 45742 | ||
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 99.2 | 96.3 | 100 |
| Redundancy | 3.4 | 3.4 | 3.4 |
| CC(1/2) | 0.998 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 80 mM HEPES (pH 7.5), 9.6% (w/v) PEG6000, 1.6% (v/v) MPD, 60 mM sodium citrate, and 80 mM NaI |
