5CRU

Crystal structure of the Bro domain of HD-PTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2014-06-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97949
Spacegroup name	P 1
Unit cell lengths	64.990, 66.874, 82.424
Unit cell angles	89.95, 89.94, 89.84

Refinement procedure

Resolution	30.975 - 2.400
R-factor	0.2215
Rwork	0.220
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rau
RMSD bond length	0.005
RMSD bond angle	1.012
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	55835
<I/σ(I)>	24.6	3.3
Completeness [%]	98.3	97.9
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	0.03 M HEPES sodium, 16%(w/v) polyethylene glycol 3350, 0.67%(w/v) tryptone, 0.03 M urea