5CRJ
Crystal Structure of the MTERF1 F322A substitution bound to the termination sequence.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.245, 90.136, 161.431 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.720 - 2.590 |
| R-factor | 0.2108 |
| Rwork | 0.208 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mva |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.593 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 161.431 | 2.598 |
| High resolution limit [Å] | 2.589 | 2.589 |
| Rmerge | 0.042 | 0.825 |
| Number of reflections | 20665 | |
| <I/σ(I)> | 29.1 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M Sodium Acetate, 0.1M Tris HCl pH 8.0, 15.5% Peg4000 |
