5CRH
Human skeletal calsequestrin, M53T mutant high-calcium complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-07-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.681, 68.553, 99.262 |
| Unit cell angles | 90.00, 92.84, 90.00 |
Refinement procedure
| Resolution | 49.570 - 2.030 |
| R-factor | 0.1765 |
| Rwork | 0.176 |
| R-free | 0.20740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5crd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.815 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9-1692) |
| Refinement software | PHENIX ((1.10-2152_1692: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.570 |
| High resolution limit [Å] | 2.030 |
| Number of reflections | 57089 |
| <I/σ(I)> | 19 |
| Completeness [%] | 100.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M MOPS, 27.5 % (v/v) 2-methyl-2,4-pentanediol, 0.2 M NaCl |
