5CRE

Human skeletal calsequestrin, D210G mutant low-calcium complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-02-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	66.106, 82.815, 89.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.716 - 3.315
R-factor	0.2531
Rwork	0.249
R-free	0.29040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5crd
RMSD bond length	0.003
RMSD bond angle	0.513
Data reduction software	HKL-2000
Phasing software	PHENIX (1.9-1692)
Refinement software	PHENIX (1.10-2152_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.716
High resolution limit [Å]	3.315
Number of reflections	7649
<I/σ(I)>	31.4
Completeness [%]	99.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M MOPS, 27.5 % (v/v) 2-methyl-2,4-pentanediol, 0.2 M NaCl