5CRC

Structure of the SdeA DUB Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	36.542, 63.340, 136.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.955 - 2.853
R-factor	0.2804
Rwork	0.280
R-free	0.29570
RMSD bond length	0.006
RMSD bond angle	1.474
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.960
High resolution limit [Å]	2.850
Rmerge	0.264
Number of reflections	7153
<I/σ(I)>	11.1
Completeness [%]	90.8
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M Tris:HCl, 3.0 M Sodium Chloride, 3% Methyl-2,4-Pentanediol