5CRB

Crystal Structure of SdeA DUB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-26
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	P 21 21 21
Unit cell lengths	62.410, 76.355, 88.429
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.078 - 2.000
R-factor	0.2052
Rwork	0.201
R-free	0.25870
RMSD bond length	0.008
RMSD bond angle	1.112
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	29618
<I/σ(I)>	13.4
Completeness [%]	97.8
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2 M potassium chloride, 0.05 M HEPES:NaOH pH 7.5, 35% (v/v) pentaerythritol/propoxylate