5CR2
E. coli MazF in complex with single strand DNA substrate analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 113.960, 113.960, 47.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.808 - 2.900 |
| R-factor | 0.234 |
| Rwork | 0.223 |
| R-free | 0.29010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mdx |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.730 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.801 | 3.170 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 7461 | |
| <I/σ(I)> | 8.96 | 2.25 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 8.95 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.17 M Sodium acetate trihydrate, 0.085 M Tris hydrocloride pH 8.5, 25.5% w/v PEG4000, 15% Glycerol |
