5CQX
E. coli MazF mutant E24A in complex with MazE residues 68-82 form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980100 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.760, 32.250, 86.260 |
| Unit cell angles | 90.00, 97.66, 90.00 |
Refinement procedure
| Resolution | 42.745 - 1.630 |
| R-factor | 0.1776 |
| Rwork | 0.174 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ub4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.051 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.740 | 1.730 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.730 | |
| Number of reflections | 22847 | |
| <I/σ(I)> | 11.1 | 2.11 |
| Completeness [%] | 95.9 | 90.9 |
| Redundancy | 3.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 0.2M Magnesium Chloride, 0.1M Sodium Cacodylate pH6.5, 50% v/v PEG6000 |
