5CQQ
Crystal structure of the Drosophila Zeste DNA binding domain in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.007, 59.705, 202.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.457 - 3.100 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26020 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.049 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.088 | 0.040 | 0.401 |
| Rmeas | 0.150 | ||
| Rpim | 0.030 | 0.019 | 0.081 |
| Total number of observations | 69974 | 9776 | 49828 |
| Number of reflections | 12079 | ||
| <I/σ(I)> | 10.6 | 30.8 | 5.5 |
| Completeness [%] | 98.7 | 90.7 | 100 |
| Redundancy | 5.8 | 4.9 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 289 | 10 mM sodium citrate, pH 4.2, 24% PEG 10000 |
