5CPU
Crystal structure of murine polyomavirus PTA strain VP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 219.610, 219.610, 99.820 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.150 - 1.640 |
R-factor | 0.1589 |
Rwork | 0.159 |
R-free | 0.17300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vpn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.155 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.680 |
High resolution limit [Å] | 1.640 | 1.640 |
Number of reflections | 334981 | |
<I/σ(I)> | 10.5 | 2.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.1 M HEPES, pH 7.5 1 M sodium phosphate monobasic 0.8 M potassium phosphate dibasic |