5CPK
Nucleosome containing methylated Sat2L DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.197, 109.297, 173.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.915 - 2.632 |
| R-factor | 0.2258 |
| Rwork | 0.224 |
| R-free | 0.28420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ut9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.630 |
| Number of reflections | 59234 |
| <I/σ(I)> | 14.1 |
| Completeness [%] | 98.8 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | potassium cacodylate, potassium chloride, manganese chloride |
