5CPJ

Nucleosome containing methylated Sat2R DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-10
Detector	RAYONIX MX225HE
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	103.452, 108.990, 173.446
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.903 - 3.150
R-factor	0.2387
Rwork	0.235
R-free	0.29550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3afa
RMSD bond length	0.010
RMSD bond angle	1.331
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.150
Rmerge	0.087
Number of reflections	34975
<I/σ(I)>	11.6
Completeness [%]	99.3
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride