5COG
Crystal structure of Yeast IRC4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.570, 38.690, 67.100 |
| Unit cell angles | 90.00, 108.59, 90.00 |
Refinement procedure
| Resolution | 63.599 - 1.613 |
| R-factor | 0.1939 |
| Rwork | 0.192 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5civ |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.972 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.600 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.071 | 0.576 |
| Number of reflections | 45428 | |
| <I/σ(I)> | 9.8 | 2.1 |
| Completeness [%] | 97.9 | 98.6 |
| Redundancy | 4.2 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 90 mM Sodium nitrate, 90 mM Sodium phosphate dibasic, 90 mM Ammonium sulfate, 0.1 M Sodium HEPES, 0.1 M MOPS, pH 7.5, 12% PEG 550 MME, 6% PEG 20,000 |
