5CO3
Crystal structure of a novel disulfide oxidoreductase from Deinococcus radiodurans crystallized in presence of DTT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | RRCAT INDUS-2 BEAMLINE PX-BL21 |
| Synchrotron site | RRCAT INDUS-2 |
| Beamline | PX-BL21 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-29 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 47.729, 63.520, 86.247 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.158 - 1.650 |
| R-factor | 0.1876 |
| Rwork | 0.186 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cnw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.867 |
| Data reduction software | Aimless |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.160 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.034 | 0.574 |
| Number of reflections | 31826 | |
| <I/σ(I)> | 23.5 | 2.2 |
| Completeness [%] | 98.7 | 88.7 |
| Redundancy | 5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 8000, Glycerol, Sodium acetate |
