5CO0

Crystal Structure of the MTERF1 Y288A substitution bound to the termination sequence.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	C 2 2 21
Unit cell lengths	87.537, 90.440, 160.514
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.260 - 2.650
R-factor	0.2091
Rwork	0.206
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mva
RMSD bond length	0.012
RMSD bond angle	1.652
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.260	80.260	2.840
High resolution limit [Å]	2.540	5.680	2.540
Rmerge	0.058	0.019	0.712
Rpim	0.023	0.008	0.281
Total number of observations	155685	13879	44373
Number of reflections	21454
<I/σ(I)>	26.3	73.4	3
Completeness [%]	99.9	99.4	100
Redundancy	7.3	6.8	7.4
CC(1/2)	1.000	1.000	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2M Sodium Acetate, 01.M Tris HCl pH 8.0, 15.5% PEG4000