5CNM

mGluR3 complexed with glutamate analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-11
Detector	RAYONIX MX225HE
Wavelength(s)	0.97949
Spacegroup name	P 21 21 2
Unit cell lengths	84.892, 99.025, 71.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.170 - 2.840
R-factor	0.1697
Rwork	0.168
R-free	0.22580
RMSD bond length	0.010
RMSD bond angle	1.180
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	71.170
High resolution limit [Å]	2.840
Number of reflections	14641
<I/σ(I)>	7.6
Completeness [%]	99.7
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	100mM Hepes pH 7.7 + 19% PEG 8K + 50mM Magnesium Sulfate