5CNL

Crystal structure of an IcmL-like type IV secretion system protein (lpg0120) from Legionella pneumophila subsp. pneumophila str. Philadelphia 1 at 2.65 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-25
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.95369,0.9793,0.9791
Spacegroup name	P 65 2 2
Unit cell lengths	83.381, 83.381, 221.829
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.018 - 2.650
R-factor	0.193
Rwork	0.192
R-free	0.21940
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	SHARP
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.018	29.018	2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge		0.041	1.310
Rmeas	0.155	0.043	1.357
Rpim	0.041	0.013	0.352
Total number of observations	194866	2050	14456
Number of reflections	13863
<I/σ(I)>	14.6	36.8	2.3
Completeness [%]	99.2	93	99
Redundancy	14.1	10.8	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.085M HEPES pH 7.5, 15% glycerol, 1.7% polyethylene glycol 400, 1.7M ammonium sulfate