5CNA

REFINED STRUCTURE OF CONCANAVALIN A COMPLEXED WITH ALPHA-METHYL-D-MANNOPYRANOSIDE AT 2.0 ANGSTROMS RESOLUTION AND COMPARISON WITH THE SACCHARIDE-FREE STRUCTURE

Replaces:  4CNA

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	123.700, 128.600, 67.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.199
Rwork	0.199
RMSD bond length	0.013
RMSD bond angle	3.150
Phasing software	X-PLOR (2.1)
Refinement software	X-PLOR (2.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.076 *
Total number of observations	62198 *
Number of reflections	58871 *
Completeness [%]	82.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	80-150 (mg/ml)
2	1	2	PIPES	0.05 (M)
3	1	2		0.1 (M)
4	1	2		1 (mM)
5	1	2		1 (mM)
6	1	2		2 (mg/ml)
7	1	2	methyl alpha-D-mannopyranoside	0.1 (M)